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Aspirin in CVD primary prevention: sex and baseline risk matter
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Comment
The Lancet, 30 May 2009
Ale Algra a b, Jacoba P Greving b
Department of Neurology, Rudolf Magnus Institute of Neuroscience, University Medical Centre Utrecht, Utrecht, Netherlands
b Julius Center for Health Sciences and Primary Care, University Medical Centre Utrecht, PO Box 85500, 3508 GA Utrecht, Netherlands
In The Lancet today, the Antithrombotic Trialists' (ATT) Collaboration reports on an individual participant data meta-analysis of primary prevention with aspirin.1 The ATT investigators should be commended for this labour-intensive work on more than 95 000 individuals from six randomised clinical trials. The main added value of the present report compared with an earlier aggregate data meta-analysis on the same six trials is that it had access to individual participant data,2 which allowed the ATT investigators to estimate the magnitude of several risk factors for selected outcomes. Factors such as age, male sex, diabetes mellitus, and high blood pressure were not only associated with ischaemic complications, but also with bleeding, although somewhat less strongly. The ATT investigators reported that the risk of serious vascular events dropped from 0·57% to 0·51% per year by the use of aspirin, but that it increased the risk of major bleeds from 0·07% to 0·10% per year. They therefore concluded that aspirin is of uncertain value for primary prevention.
A major difference in reporting between the two studies is that the investigators of the aggregate data meta-analysis primarily reported effectiveness data for women and men separately, whereas the present report does not. The ATT investigators do provide these data, but reasoned that the proportional reduction in specific vascular outcomes did not differ significantly between men and women when adjustment for multiple comparisons was made. Hence, they do not differentiate between women and men in their recommendations.
Is this the optimum approach? First, at the level of single comparisons there were several tests for the different effects of aspirin in women and men that were significant. For major coronary events, the p value for heterogeneous effectiveness between the sexes was 0·03, that for first stroke (any type) was 0·004, and that for probable ischaemic stroke was 0·005. The latter two remained significant when the ATT global test for heterogeneity was used. The ATT investigators, however, dismissed these differences with the use of a strict multiple comparisons criterion, whereas published work for multiple comparisons is at least as heterogeneous itself.3-5 Second, the ATT investigators obtained information from secondary prevention trials in which different effects of aspirin between the sexes were virtually absent. We think that one should be very cautious with borrowing of data from patients with clinically manifest disease. We are therefore not willing to give the secondary prevention data as much weight in the assessment of the primary prevention data as the ATT investigators do. Third, incidence rates of myocardial infarction, ischaemic stroke, intracranial haemorrhage, and gastrointestinal bleeding differ between the sexes, which affects the balance between benefits and risks.
How can we use today's study in daily practice? For example, should the first author of this Comment (55-year-old man, slightly elevated cholesterol, no other vascular risk factors) take an aspirin a day to keep the doctor away? The ATT investigators try to balance benefits (less serious vascular events) and risks (more haemorrhages), but without taking into account the consequences of both harms and benefits on quality of life.
We thus decided to update our previous cost-effectiveness analysis6 with the new data provided by the ATT investigators. We have used the slightly different risk ratios for cardiac events and ischaemic stroke for women and men separately. Moreover, in individuals for whom we used an increased risk of cardiovascular disease we let the risk of haemorrhage increase proportionally on the basis of the new ATT observations. The table summarises for which individuals an incremental cost-effectiveness ratio of 20 000 or less would be obtained; these individuals are probably the best candidates for prevention with aspirin. These ratios are the most reliable estimates since all data have been incorporated in the cost-effectiveness analysis, but these ratios come with their own uncertainty (see the sensitivity analysis in table 5 of our previous report).6 The new estimates from the cost-effectiveness analysis imply that the first author does not need to take an aspirin a day. It should be emphasised that our model also took the (dis)utility of taking aspirin into account and our sensitivity analyses indicated that the results of our analyses were sensitive to it. Patients might not wish to be medicalised7-such considerations are important in the decision to take aspirin or not. Whether statins should be preferred above aspirin is a different and difficult question that needs careful consideration too. Apart from drug treatment, one must not forget the importance of lifestyle changes, such as cessation of smoking, healthy diet, and regular exercise.
Editors' note: Aspirin is enormously useful as a prophylactic for cardiovascular events including myocardial infarction and ischaemic stroke. There has been concern, however, that aspirin can also increase haemorrhagic strokes and cause gastrointestinal bleeding. This study investigated the balance of positive and negative effects, and the results indicate no overwhelming difference. For individual patients, therefore, it depends on whether it is better to risk an MI or a gastrointestinal bleed.
"Aspirin in the primary and secondary prevention of vascular disease: collaborative meta-analysis of individual participant data from randomised trials"
Antithrombotic Trialists' (ATT) Collaboration
Summary
Background
Low-dose aspirin is of definite and substantial net benefit for many people who already have occlusive vascular disease. We have assessed the benefits and risks in primary prevention.
Methods
We undertook meta-analyses of serious vascular events (myocardial infarction, stroke, or vascular death) and major bleeds in six primary prevention trials (95 000 individuals at low average risk, 660 000 person-years, 3554 serious vascular events) and 16 secondary prevention trials (17 000 individuals at high average risk, 43 000 person-years, 3306 serious vascular events) that compared long-term aspirin versus control. We report intention-to-treat analyses of first events during the scheduled treatment period.
Findings
In the primary prevention trials, aspirin allocation yielded a 12% proportional reduction in serious vascular events (0·51% aspirin vs 0·57% control per year, p=0·0001), due mainly to a reduction of about a fifth in non-fatal myocardial infarction (0·18% vs 0·23% per year, p<0·0001). The net effect on stroke was not significant (0·20% vs 0·21% per year, p=0·4: haemorrhagic stroke 0·04% vs 0·03%, p=0·05; other stroke 0·16% vs 0·18% per year, p=0·08). Vascular mortality did not differ significantly (0·19% vs 0·19% per year, p=0·7). Aspirin allocation increased major gastrointestinal and extracranial bleeds (0·10% vs 0·07% per year, p<0·0001), and the main risk factors for coronary disease were also risk factors for bleeding. In the secondary prevention trials, aspirin allocation yielded a greater absolute reduction in serious vascular events (6·7% vs 8·2% per year, p<0.0001), with a non-significant increase in haemorrhagic stroke but reductions of about a fifth in total stroke (2·08% vs 2·54% per year, p=0·002) and in coronary events (4·3% vs 5·3% per year, p<0·0001). In both primary and secondary prevention trials, the proportional reductions in the aggregate of all serious vascular events seemed similar for men and women.
Interpretation
In primary prevention without previous disease, aspirin is of uncertain net value as the reduction in occlusive events needs to be weighed against any increase in major bleeds. Further trials are in progress.
Funding
UK Medical Research Council, British Heart Foundation, Cancer Research UK, and the European Community Biomed Programme.
Introduction
In patients who are at high risk because they already have occlusive vascular disease, long-term antiplatelet therapy (eg, with aspirin) reduces the yearly risk of serious vascular events (non-fatal myocardial infarction, non-fatal stroke, or vascular death) by about a quarter.1, 2 This decrease typically corresponds to an absolute reduction of about 10-20 per 1000 in the yearly incidence of non-fatal events, and to a smaller, but still definite, reduction in vascular death. Against this benefit, the absolute increase in major gastrointestinal or other major extracranial bleeds is an order of magnitude smaller. Hence, for secondary prevention, the benefits of antiplatelet therapy substantially exceed the risks.
For primary prevention, however, the balance is less clear because the risks without aspirin, and hence the absolute benefits of aspirin, are generally an order of magnitude lower than in secondary prevention. Previous meta-analyses of primary prevention trials were not based on individual participant data, so they could not compare reliably the benefits and risks of aspirin in prognostically important groups (such as older people and others at increased risk of coronary heart disease), and could not quantify reliably the extent to which people at increased risk of coronary heart disease might also be at increased risk of bleeding. Current guidelines largely ignore any differences in bleeding risk, and recommend that aspirin be used widely for primary prevention in those at moderately raised risk of coronary heart disease.3-5 It has also been suggested that, since age is a major determinant of the risk of coronary heart disease, daily aspirin should be started in all people above a specific age, either alone or in combination with other drugs.6-8
The alternative to primary prevention is deferral of the start of long-term aspirin until some evidence of occlusive vascular disease is noted. The main disadvantage of deferral is that the first manifestation of disease might be a disabling or fatal event, but the main advantage is that it could avoid decades of slightly increased risk of cerebral haemorrhage or major extracranial bleeding. In the primary prevention trials, most controls who had a non-fatal myocardial infarction or occlusive stroke while not on aspirin would probably then have started long-term aspirin to avoid recurrence, so the mortality results from those trials can help to decide between the policies of immediate versus deferred aspirin (ie, deferral of the start of long-term aspirin until there is evidence of disease).
In view of the limitations of the analyses underlying current guidelines, and the large populations affected by these guidelines, a collaborative meta-analysis of individual participant data was established involving the principal investigators of all large trials of primary prevention with aspirin. Meta-analyses of previously obtained individual participant data from 16 secondary prevention trials of aspirin were also undertaken to compare the proportional and absolute effects of aspirin in these two treatment settings.1, 2
Results
Six primary prevention trials were available (95 000 individuals, 3554 serious vascular events; table 1, figure 1).9-14 One12 recruited people with hypertension, and two9, 13 recruited people with coronary risk factors (although without overt disease). The results were contrasted with those from the 16 secondary prevention trials (17 000 individuals, 3306 vascular events).2
In the primary prevention trials, 1671 serious vascular events occurred during 330 000 person-years (0·51% per year) in people allocated aspirin compared with an adjusted total of 1883 events during 330 000 person-years (0·57% per year) in those allocated control. This small absolute reduction (only 0·07% per year) represented a 12% proportional reduction (rate ratio [RR] 0·88 [95% CI 0·82-0·94], p=0·0001; figure 1), with no significant heterogeneity between the prespecified subgroups (global test for heterogeneity p=0·7; figure 2). The proportional reduction seemed similar (p=0·9) in men and women, and did not differ significantly (p trend=0·3) between those with predicted 5-year risk of coronary heart disease less than 2·5%, 2·5-5%, 5-10%, or 10% or more (figure 2). The apparently unpromising result in the 2% of all participants in the primary prevention trials who were in the highest risk group was statistically unreliable because it involved small numbers (131 [3·59% per year] vs 127 [3·44% per year] vascular events; RR 1·07 [99% CI 0·76-1·50], p=0·6; figure 2). The most important predictor of risk was age; the mean age at entry for those in the highest risk group was 69 years (SD 6) (so their mean age during the trials was over 70 years). The proportional reduction in risk of any serious vascular event did not differ significantly between primary and secondary prevention trials, but the absolute risk reduction was much smaller in primary than in secondary prevention (table 2).
Since major coronary events and strokes accounted for a large proportion of serious vascular events, the effects of aspirin on each outcome were assessed separately. In the primary prevention trials, allocation to aspirin yielded an 18% proportional reduction in major coronary events, but only a small absolute reduction (0·28% vs 0·34% per year; RR 0·82 [95% CI 0·75-0·90], p<0·0001; figure 1). Most of this decrease derived from a 23% proportional reduction in non-fatal myocardial infarction (0·18% vs 0·23% per year; RR 0·77 [99% CI 0·67-0·89], p<0·0001; figure 1), with no clear reduction in mortality from coronary heart disease (0·11% vs 0·12% per year; RR 0·95 [99% CI 0·78-1·15], p=0·5), although the CI for the proportional reduction in mortality from coronary heart disease is wide. The proportional reduction in major coronary events seemed to be similar in primary and secondary prevention (RR 0·82 [95% CI 0·75-0·90] primary and RR 0·80 [0·73-0·88] secondary), but the absolute benefit differed by an order of magnitude (absolute benefits 0·06% per year primary and 1·00% per year secondary) (table 2).
For major coronary events in the primary prevention trials, the 11 separate tests for trend or heterogeneity of effect yielded only one, between men and women, that was marginally significant (p=0·03; figure 3 and webappendix p 4) if considered in isolation, but it was no longer significant if multiplied by 11 to allow for multiple comparisons (p=0·33). Furthermore, the 16 secondary prevention trials also suggested no heterogeneity of the effect on major coronary events between men and women (figure 3). Conversely, for ischaemic stroke, the six primary prevention trials suggested a greater proportional risk reduction in women than in men (p=0·08 for heterogeneity of effect between men and women if considered in isolation, but p=0·88 [not significant] if multiplied by 11 to allow for multiple comparisons); again, however, the 16 secondary prevention trials suggested no such heterogeneity of effect. For the aggregate of all serious vascular events, gender was of no apparent relevance in either type of trial to the proportional reduction produced by allocation to aspirin (figure 3).
Aspirin seemed to increase the incidence of haemorrhagic stroke both in the primary and in the secondary prevention trials (p=0·05 and p=0·07, respectively; p=0·01 when analysed together). Conversely, aspirin seemed to reduce the incidence of ischaemic stroke in both types of trial (p=0·05 and p=0·04, respectively; p=0·005 when analysed together). The proportion of strokes of known cause that were haemorrhagic was greater in the primary than in the secondary prevention trials (23% vs 15%; table 2); this was probably also true for strokes of unknown cause. For, at least 84% (893/1060: webappendix p 18) of the strokes in the secondary prevention trials were in patients with a previous history of ischaemic stroke or transient cerebral ischaemia, who would be at high risk of recurrence.
In the primary prevention trials, aspirin had no net effect on strokes of known cause (haemorrhagic plus ischaemic), on strokes of unknown cause, or on the aggregate of all strokes (table 2). In the secondary prevention trials, however (in which a smaller proportion of the strokes of known cause were haemorrhagic), aspirin significantly reduced the aggregate of all strokes.
As most strokes do not cause death, and haemorrhagic strokes may be more dangerous than ischaemic strokes, the proportional effects of aspirin on overall stroke mortality and on non-fatal stroke could differ. In figure 4 the results are therefore subdivided not only by cause but also by outcome (any outcome, or fatal). For strokes with any outcome (figures 4A, 4C, 4E, and 4G) the results are those from table 2. The subtotals in figure 4 combine the primary and secondary trial results. Taking both types of trial together, there was evidence of an adverse effect on haemorrhagic stroke (RR 1·39 [95% CI 1·08-1·78], p=0·01; figure 4A) but a protective effect on ischaemic stroke (RR 0·83 [0·73-0·95], p=0·005; figure 4C).
If attention is restricted to the fatal strokes just in the primary prevention trials, then fatal haemorrhagic strokes outnumber fatal ischaemic strokes (82 vs 53) and there is, if anything, an adverse effect on overall stroke mortality (119 vs 98 fatal strokes, p=0·18 [non-significant]; figure 4H). For, there was a significant excess of fatal haemorrhagic strokes in participants allocated aspirin (52 vs 30; RR 1·73 [99% CI 0·96-3·13], p=0·02; figure 4B) plus similar numbers of other fatal strokes (24 vs 29 ischaemic plus 43 vs 39 unknown cause; figures 4D and 4F).
Since allocation to aspirin had no significant effect on fatal stroke, fatal coronary heart disease or other vascular causes of death, there was no significant reduction in overall vascular mortality in the primary prevention trials (RR 0·97 [95% CI 0·87-1·09], p=0·7; figure 1). Since there was also no significant effect on non-vascular mortality (RR 0·93 [95% CI 0·85-1·02], p=0·1) or on mortality from unknown causes (RR 0·96 [99% CI 0·70-1·30], p=0·7), there was no significant effect on total mortality (RR 0·95 [95% CI 0·88-1·02], p=0·1; figure 5). By contrast, in the secondary prevention trials, aspirin seemed to reduce vascular mortality (RR 0·91 [0·82-1·00], p=0·06) and had no significant effect on other mortality (RR 0·85 [0·66-1·08], p=0·2), yielding a 10% reduction in total mortality (RR 0·90 [0·82-0·99], p=0·02).
The main hazard of aspirin is haemorrhage and, apart from any effect on intracerebral haemorrhage, aspirin increased major gastrointestinal and other extracranial bleeds by about half in the primary prevention trials (0·10% vs 0·07% per year; RR 1·54 [1·30-1·82], p<0·0001; table 2 and webappendix pp 9, 10). This increase was non-significantly greater in participants with high cholesterol (p=0·02 for trend if considered in isolation, but p=0·22 if multiplied by 11; webappendix p 10). The excess risk was chiefly of non-fatal bleeds; perhaps by chance, there were actually fewer fatal bleeds in participants allocated aspirin than in controls (nine vs 20; figure 5). Major bleeds were recorded in only five of the 16 secondary prevention trials, so a meta-analysis might be unreliable. There was again, however, a significant excess of major bleeds among those allocated aspirin (RR 2·69 [1·25-5·76], p=0·01; table 2 and webappendix p 20), with no significant heterogeneity between the relative risks in the six primary and these five secondary prevention trials (p=0·2; table 2).
The absolute yearly incidence of vascular events and of major extracranial bleeds varied substantially among participants in the primary prevention trials. Poisson regression in 93 918 individuals without known vascular disease at entry within the primary prevention trials indicated that age (per decade), male sex, diabetes, current smoking, and mean blood pressure (per 20 mm Hg) were each associated with about a two-fold increased risk of major coronary events, whereas total cholesterol (per 1 mmol/L) and body-mass index (per 5 kg/m2) were more weakly associated with such events (table 3). Measurements of cholesterol fractions were not sought, and body-mass index acts mainly as a determinant of other cardiac risk factors15 so is of little independent relevance in these multivariate analyses. The main risk factors for coronary events in table 3 were also associated with haemorrhagic events, although for most the associations were slightly weaker for bleeding than for occlusive events.
Discussion
Previous meta-analyses have shown that aspirin is of substantial net benefit in secondary prevention,1, 2 but the balance of beneficial effects and bleeding hazards in primary prevention was less clear. The availability of individual participant data for the present meta-analysis has allowed more reliable comparison of the benefits and hazards of aspirin in apparently healthy people. All four of the proportional reductions in major coronary events and in ischaemic stroke in the primary and in the secondary prevention trials were similar to each other (figure 3). Vascular mortality was not significantly reduced in the primary prevention trials, although a proportional reduction comparable with that in the secondary prevention trials could not be excluded. Whether or not the proportional benefits are similar, however, the absolute benefits of aspirin are an order of magnitude smaller in the primary than in the secondary prevention trials (table 2). (We have ignored the hypothesis16, 17 of an eventual reduction in cancer mortality, since it would be expected to have little effect on our analyses of mortality during the scheduled treatment period.)
In the primary prevention trials, the proportional reduction in serious vascular events did not depend significantly on age or sex (and the suggestion, on the basis of the primary prevention trials,18 that the proportional reductions in particular vascular outcomes might differ between men and women was not supported by the secondary prevention trials; figure 3). Nor did it depend significantly on smoking history, blood pressure, total cholesterol, body-mass index, history of diabetes, or predicted risk of coronary heart disease. In particular, there was no significant trend in the proportional effects of aspirin in people at very low, low, moderate, and high estimated risk of coronary heart disease. If the proportional risk reductions in these different subgroups really are similar, then the absolute risk reductions will depend chiefly on an individual's absolute risk without treatment.
Figures 6 and 7 provide hypothetical calculations of what the absolute effects of aspirin allocation on 5-year outcome would be (in the absence of non-vascular causes of death) if the yearly event rates and the proportional effects of aspirin were as in the primary and as in the secondary prevention trials, and if these proportional effects were independent of age, sex, and other risk factors. Long-term low-dose aspirin had significant effects on both fatal and non-fatal events in people who already had occlusive vascular disease (table 2). Figure 6 suggests that, in this secondary prevention setting, aspirin would be of substantial net benefit (irrespective of age or sex), that it would reduce non-fatal vascular events by much more than it would increase major extracranial bleeds, and that-despite any adverse effects on cerebral haemorrhage-it would reduce overall vascular mortality (a result that is strongly reinforced by meta-analyses of all of the trials of any antiplatelet regimen in secondary prevention1, 2). Nowadays, however, many patients with a history of occlusive stroke or myocardial infarction would have their risks of recurrence reduced substantially by statins, other modern drugs, and, when appropriate, vascular procedures. For occlusive vascular events, the relative risks produced by these other interventions and by aspirin might well be approximately multiplicative. If so, and if the other interventions approximately halve the recurrence risk, then the absolute benefit of adding aspirin to these other methods might be only about half as great as that of giving aspirin alone. Still, figure 6 suggests that, for secondary prevention, the net benefits of adding aspirin would substantially exceed the bleeding hazards, irrespective of age or sex.
In the primary prevention trials, however, the absolute risk of a serious vascular event among people of a given age and sex was an order of magnitude less than in the secondary prevention trials. Figure 6 suggests that (irrespective of age or sex) the absolute reduction in occlusive events would be only about twice as large as the absolute increase in bleeding. Moreover, these trials of aspirin were mainly in people who were not taking statin therapy, which would have reduced both myocardial infarction and ischaemic stroke with little hazard.19, 20 Generic statins are now widely available at low cost and, because of their efficacy and safety, primary prevention by a statin could well be preferred to primary prevention only by aspirin. If so, then one of the main questions for aspirin in primary prevention nowadays is whether it is worthwhile to add it to a statin (or to some statin-based combination of measures). If the risk of occlusive vascular disease is already approximately halved by statins or other measures, then the further absolute benefit of adding aspirin could well be only about half as large as was suggested by these primary prevention trials, but the main bleeding hazards could well remain. In that case, the benefits and hazards of adding long-term aspirin in people without pre-existing disease might be of approximately similar magnitude.
There is, of course, still the possibility that the primary prevention trials have, by chance, somewhat underestimated the main effects on mortality in the populations they studied (as is shown by the CIs in figure 5). There is also the possibility that some particular category of individuals will eventually be identified in which primary prevention with aspirin is of definite net benefit. One particularly important such category might be adults with diabetes but no known vascular disease, for whom aspirin is at present recommended.21 Although the evidence from the six primary prevention trials reviewed here is consistent with some net benefit in such patients (figure 2), the evidence from three other primary prevention trials in diabetes has been unpromising.22-24 Two much larger trials are, however, now recruiting only patients with diabetes.25, 26 More generally only 9% of participants in the six primary prevention trials had predicted coronary heart disease incidence rates above 1% per year, so the present results among them are not, on their own, reliable (figure 2). Two major new trials in such moderately high-risk individuals are, however, now being undertaken,27, 28 which will eventually yield more reliable evidence.
To maximise the excess of benefit over hazard in primary prevention, most current guidelines3-5 recommend that aspirin be given to those with risk of coronary heart disease exceeding a particular threshold. These guidelines implicitly assume, however, either that the absolute risk of bleeding remains approximately constant irrespective of risk of coronary heart disease,4, 5 or that it depends solely on age,3 whereas the present analyses showed that other risk factors for this disease are also risk factors for bleeding (table 3). As a result, even for people at moderately increased risk of coronary heart disease, the major absolute benefits and hazards of adding aspirin to a statin-based primary prevention regimen could still be approximately evenly balanced, as is suggested by the calculations in figure 7.
A non-fatal stroke or heart attack is more likely to result in long-term disability than is a non-fatal gastrointestinal (or other extracranial) bleed, but in primary prevention the net absolute reduction in disabling or fatal occlusive events is likely to be small, and at least partially offset by a small increase in serious intracranial and extracranial bleeds. Thus, although it might cost little to add aspirin to any other drugs that are being used for the primary prevention of vascular disease, the additional effectiveness against fatal or disabling outcomes has not been reliably demonstrated for men or women of any age who do not yet have any relevant disease (and, if effectiveness is uncertain then detailed estimates of cost-effectiveness29 are of limited relevance). Moreover, drug safety (like vaccine safety) is of particular importance in public health recommendations for large, apparently disease-free populations; there should be good evidence that benefits exceed risks by an appropriate margin. Hence, although the currently available trial results could well help inform personally appropriate judgments by individuals about their own use of long-term aspirin, they do not seem to justify general guidelines advocating the routine use of aspirin in all apparently healthy individuals above a moderate level of risk of coronary heart disease.3-8
Methods
Trial eligibility
Primary or secondary prevention trials were eligible only if they involved a randomised comparison of aspirin versus no aspirin (with no other antiplatelet drug in either group). Primary prevention trials excluded individuals with any history of occlusive disease at entry. (Subsequent enquiry showed that 2% did in fact have some evidence of previous vascular disease, but they remain in all analyses apart from those estimating the absolute effects of aspirin.) Primary prevention trials were sought only if they recruited at least 1000 non-diabetic participants with at least 2 years of scheduled treatment. Individual participant data were provided from all six published trials.9-14 Unpublished trials were sought through electronic searches and discussions, but none was identified.
Secondary prevention trials were included in analyses if they involved individuals with previous myocardial infarction (six trials) or stroke or transient cerebral ischaemia (ten trials), and had contributed individual participant data to the 2002 Antithrombotic Trialists' (ATT) report (webappendix pp 11-23).1, 2 Two further trials for which only tabular data were available are shown in the webappendix but do not contribute to analyses. Electronic searches established that no similar trials of aspirin had been reported since 2002.
Prespecified analyses
The comparisons were intention-to-treat analyses of first events during the scheduled treatment period in all participants allocated aspirin versus all those allocated control (irrespective of any other treatment allocated factorially). The main outcomes were serious vascular event, defined as myocardial infarction, stroke, or death from a vascular cause (including sudden death, pulmonary embolism, haemorrhage, and, for secondary prevention trials only, death from an unknown cause); major coronary event (myocardial infarction, coronary death, or sudden death); any stroke (haemorrhagic or probably ischaemic [ie, definitely ischaemic or of unknown type]); death from any cause; and major extracranial bleed (mainly gastrointestinal and usually defined as a bleed requiring transfusion or resulting in death). In the primary prevention trials, myocardial infarctions and strokes were classified as fatal or non-fatal in accordance with each trial's definitions. In the secondary prevention trials, as previously,2 these outcomes were regarded as non-fatal only if the patient was alive at the end of the trial or died of a non-vascular cause. Five of the primary prevention trials classified stroke subtypes on the basis of either clinical examination10 or CT imaging.9, 11, 13, 14 In the sixth trial,12 imaging information was available only for strokes that had been confirmed as cerebral bleeds. In most secondary prevention trials little information about stroke causes was available (webappendix pp 15-18).1, 2
Statistical analysis
The log-rank observed minus expected (o-e) statistics, one from each trial, and their variances (v), were summed to produce, respectively, a grand total observed minus expected (G) and its variance (V). The one-step estimate of the log of the event rate ratio is G/V. The χ2 test statistic (χ2n-1) for heterogeneity between n trials is S-(G2/V), where S is the sum over all the trials of (o-e)2/v. Heterogeneity of rate ratios among multiple subgroups defined by baseline characteristics was investigated by a global heterogeneity test, which helps to avoid misinterpreting false positive results arising from multiple comparisons. (For each characteristic [eg, age] a χ2 test for trend on 1 degree of freedom was calculated. If there are 11 different χ2 tests and none of the trends is real, then their sum has expectation 11 and has variance at least as great as that of χ211.)
For trials that randomised unequally (ie, 2:1), we multiplied the control group by two when displaying adjusted control totals and describing the total amount of information available, but not in other calculations. For the purposes of discussion, we calculated what the absolute effects of aspirin allocation would be on outcome at 5 years (only two trials9, 14 had much longer follow-up) if the yearly event rates were constant and the proportional effects of aspirin were independent of age, sex, and other risk factors.
To identify risk factors for various outcomes in people in the primary prevention trials without any known history of vascular disease, we used Poisson regression, stratified by trial, to estimate the common linear dependence of the log of the event rate on age, sex, diabetes, current cigarette smoking, total cholesterol, mean (of systolic and diastolic) blood pressure, body-mass index, and allocation to aspirin or control (webappendix p 24). Additionally, the results of this model for major coronary events in control participants only, together with the absolute event rates in the controls of each trial, were used to classify the baseline risks of all participants (including those allocated aspirin) as very low (predicted 5-year risk of coronary heart disease without aspirin <2·5%), low (2·5-5%), moderate (5-10%), or high (≥10%).
Further details of the trials and of the analyses are available in the webappendix.
Role of the funding sources
The sponsors of the study had no role in study design, data collection, data analysis, data interpretation, or writing of the report. The secretariat had full access to all the data in the study and the writing committee had final responsibility for the decision to submit for publication.
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