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A Vital Role for Interleukin-21 in the
Control of a Chronic Viral Infection - full text pdf attached
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John S. Yi, Ming Du, Allan J. Zajac*
"Therefore, we anticipate that the cautious development of approaches to modulate the levels of IL-21, or regulate the induction of cellular subsets that generate IL-21, will provide new therapeutic opportunities to improve immunity to diseases that require CD8+ T cell responses to be controlled, such as chronic viral infection and tumors."
Understanding the factors that regulate the induction, quality, and longevity of antiviral T cell responses is essential for devising rational strategies to prevent or combat infections. In this study, we show that interleukin-21 (IL-21), likely produced by CD4+ T cells, directly influences the generation of polyfunctional CD8+ T cells and that the number of CD4+ T cells that produce IL-21 differs markedly between acute and chronic infections. IL-21 regulates the development of CD8+ T cell exhaustion and the ability to contain chronic lymphocytic choriomeningitis virus infection. Thus, IL-21 serves as a critical helper factor that shapes the functional quality of antiviral CD8+ T cells and is required for viral control.
Originally published in Science Express on 14 May 2009
Science 19 June 2009:
Vol. 324. no. 5934, pp. 1572 - 1576
DOI: 10.1126/science.1175194
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294-2170, USA.
* To whom correspondence should be addressed. E-mail:
A hallmark of robust antiviral immunity is the induction of CD4+ and CD8+ T cell responses, which act cooperatively and in conjunction with other immune system components, to control infection. The consequences of an ineffective immune response can be catastrophic, favoring viral persistence or the erosion of long-lived immunological memory. During the initial phases of many chronic infections, including hepatitis C virus (HCV) and human immunodeficiency virus (HIV) infections, CD8+ T cell responses are induced but can fail to attain or lose the ability to elaborate key effector functions (1-9). Although the cellular and molecular cues that dictate the development of robust CD8+ T cells are not fully elucidated, in the absence of CD4+ T cell help, CD8+ T cell responses are compromised (2, 5, 6, 10-18). CD4+ T cells are the primary producers of interleukin-21 (IL-21), a member of the common-{gamma} chain family of cytokines (19-21). Functions of IL-21 are wide-ranging and include promoting B cell and antibody responses and inducing the development of Th17 and follicular helper CD4+ (Tfh) lineages (19-24). Given that CD4+ T cells are necessary for optimal antiviral CD8+ T cell responses, we investigated the role of IL-21 in CD8+ T cell responses to viral infections.
A comparative analysis of lymphocytic choriomeningitis virus (LCMV)-specific CD4+ T cells revealed marked differences in the induction of IL-21+ CD4+ T cells after acute LCMV-Armstrong (Arm) and chronic LCMV-clone 13 (Cl 13) infections of C57BL/6 (B6) mice (Fig. 1) (25). By 8 days, both infections elicited polyclonal virus-specific IL-21+ CD4+ T cells; however, this response was 7.8 times lower in the LCMV-Cl 13-infected cohort (Fig. 1B; P < 0.001). Because CD4+ T cells are obligatory for the control of LCMV-Cl 13, these results suggest that the CD4+ T cell response to this infection, albeit weak, is capable of providing some help (2, 5, 6, 10).
The pronounced IL-21+ CD4+ T cell response after acute LCMV-Arm infection prompted us to analyze whether IL-21 influenced the generation of germinal center (GC) B cells, Tfh cells, and antibody responses (fig. S1). The percentage and number of GC B cells and Tfh (ICOS+, CXCR5+) T cells as well as LCMV-specific antibody titers were similar between 8 to 9 days after LCMV-Arm infection of Il21+/+ and -/- mice. Thus, acute LCMV infection can trigger the development of these responses even in the absence of IL-21. Moreover, all cohorts successfully controlled LCMV-Arm infection because serum viral titers were below the limits of detection [<50 plaque-forming units (PFU)/ml] by this time point. Although appreciable antibody levels were detectable between days 44 to 120 after infection, endpoint titers were three times lower in Il21-/- mice.
To further analyze the role of IL-21 in promoting antiviral immunity, we evaluated the responses of Il21+/+, +/-, and -/- mice to LCMV-Cl 13 infection. Mice infected with LCMV-Cl 13 typically exhibit high-grade viremia and progressive reductions in the functional capacity of antiviral CD8+ T cells, termed exhaustion (1, 2, 5, 6, 9, 26). Eight days after LCMV-Cl 13 infection, during the effector phase, the magnitudes of the antiviral CD4+ and CD8+ T cell responses and the frequency of interferon-{gamma} (IFN-{gamma})-producing antiviral CD8+ T cells were similar among Il21+/+, +/-, and -/- cohorts (figs. S2 and S3, A and B); however, we observed differences in their functional quality (Fig. 2, A and B, and fig. S3B). Both the percentages and absolute numbers of polyfunctional, IL-2-producing CD8+ T cells were reduced in Il21-/- mice, with Il21+/- mice exhibiting an intermediate phenotype (Fig. 2, A and B, and fig. S3B). We observed similar trends for tumor necrosis factor-{alpha} (TNF-{alpha}) production (fig. S4A). Thus, IL-21 deficiency results in impaired polyfunctional effector CD8+ T cell responses during the initial phases of LCMV infection.
Given the altered cytokine responses by antiviral CD8+ T cells after LCMV-Cl 13 infection of IL21-/- mice, we next tracked viral loads over time to determine whether the absence of IL-21 compromised the containment of the infection (Fig. 2C and fig. S5). As expected, Il21+/+ hosts slowly controlled LCMV-Cl 13 infection (1, 2, 5, 9, 26). By contrast, viral titers in the serum, livers, and lungs of Il21-/- mice remained high, a phenotype similar to that observed in mice lacking CD4+ T cells (Fig. 2C and fig. S5). Il21+/- mice showed an intermediate pattern of clearance, with viral loads decreasing more slowly than in Il21+/+ mice and the infection persisting at high levels or breaking through in three of the five Il21+/- mice tested between days 136 and 148 after infection. These data suggest that IL-21 is critical for control of chronic viral infection.
Evaluation of antiviral CD8+ T cell responses at later stages after LCMV-Cl 13 infection revealed severe functional exhaustion in Il21-/- mice (Fig. 2D and figs. S3, C and D, and S4). By 136 days, Il21+/+ mice had brought the infection under control, and IFN-{gamma}, IL-2 (Fig. 2D), and TNF-{alpha} (fig. S4B) production was detectable by antiviral, primarily glycoprotein (GP)-specific, CD8+ T cells. Moreover, these antiviral CD8+ T cells expressed intermediate levels of CD43 (CD43int) and low levels of programmed death-1 (PD-1low), a phenotype more similar to resting memory T cells (Fig. 2, E and F). In Il21+/- mice, the phenotypic and functional properties of the antiviral CD8+ T cells diverged depending on their virological status. CD8+ T cells from mice able to contain the infection resembled antiviral CD8+ T cells from Il21+/+; however, in mice with high viral titers, CD8+ T cell responses were more similar to those observed in Il21-/- hosts (fig. S4B). IL-2, IFN-{gamma}, and TNF-{alpha} production by Il21-/- virus-specific CD8+ T cells were greatly reduced or absent (Fig. 2D and figs. S3D and S4B), but these T cells were CD43high, PD-1high (Fig. 2, E and F), a hallmark of the exhaustion that develops in chronically infected hosts (6, 9). Interestingly, the emergence of exhausted virus-specific CD8+ T cells in Il21-/- mice parallels what is observed after LCMV-Cl 13 infection of CD4-deficent hosts (2, 5, 6, 10). Thus, the absence of IL-21 production results in a failure to contain the infection and a silencing of antiviral CD8+ T cell functions.
We next examined whether IL-21 was acting directly to promote and sustain CD8+ T cell responses. To directly compare, within the same host, the responses and fates of cells of the immune system that can and cannot perceive IL-21-derived signals, we generated bone-marrow chimeras with use of a mixture of allelically marked Il21r+/+ (CD45.1) and Il21r-/- (CD45.2) donor cells. As a control, we also generated mixed bone-marrow chimeric mice that were reconstituted with Il21r+/+ (CD45.1) bone marrow and Il21r+/+ (CD45.2) bone marrow prepared from the littermates of Il21r-/- mice. Before infection, the ratio of CD45.1:CD45.2 CD8+ T cells was 52:48 and 70:30 in the control and experimental cohorts, respectively. Nevertheless, both Il21r+/+ and Il21r-/- virus-specific CD8+ T cells were detectable in the circulation by 8 days after LCMV-Cl 13 infection (Fig. 3). Thus, the elaboration of primary virus-specific CD8+ T cell responses can occur independently of IL-21. Further tracking of virus-specific CD8+ T cells in the circulation at 16 days after infection revealed a preferential and rapid loss of Il21r-/- antiviral CD8+ T cells. This was confirmed by enumeration of splenic responses 3 weeks after infection, which revealed a 40 times fewer number of Il21r-/- virus-specific CD8+ T cells by comparison with Il21r+/+ counterparts in the same host (Fig. 3, C and D). These data illustrate the direct requirement of IL-21 for supporting and maintaining antiviral CD8+ T cells during chronic viral infections.
We next evaluated whether the addition of IL-21 enhanced antiviral CD8+ T cells because the absence of IL-21-dependent signaling impaired these responses. We investigated whether administration of IL-21 could improve responses and viral control in Cd4-/- mice because CD4+ T cells are a principal source of this cytokine. These "helpless" mice do not usually control LCMV-Cl 13 infection and develop severe CD8+ T cell exhaustion (2, 5, 6, 10). Daily injections of recombinant IL-21 to LCMV-Cl 13-infected Cd4-/- mice enhanced the functional quality of virus-specific CD8+ T cells, particularly the nucleoprotein (NP)396 epitope-specific population, which usually rapidly succumbs to exhaustion (Fig. 4). Importantly, IL-21 treatment resulted in lower viral titers (Fig. 4C). The delicate balance between the quality and size of the antiviral immune response and the hostsŐ viral burden became apparent as 70% of the treated mice became moribund, reaching experimental endpoints requiring euthanasia. Illness and death after LCMV infection is classically associated with immunopathology (27). Thus, although IL-21 administration can improve antiviral CD8+ T cell responses and viral clearance and has been safely used in the context of tumor immunotherapy in mice and humans, care will need to be taken before applying this treatment strategy to chronic viral infections (21, 28, 29).
Collectively, our findings provide insights into the determinants of the functional quality of CD8+ T cell responses and the role of IL-21 in ensuring the successful control of infection. The results implicate IL-21 as a critical helper factor that couples the requirement for CD4+ T cells, which produce this cytokine, to the elaboration and maintenance of polyfunctional CD8+ T cells capable of clearing virus-infected cells. We observed that induction of IL-21-producing CD4+ T cells is markedly reduced during the initial phase of chronic viral infection, suggesting that this could be a general feature of infections that elicit nonprotective adaptive immune responses, such as hepatitis B virus, HCV, and HIV (8, 30). This poor induction of IL-21 may result from the failure to initiate robust CD4+ T cell responses as well as the exhaustion or the viral-induced depletion of these cells that may occur during certain chronic infections. Therefore, we anticipate that the cautious development of approaches to modulate the levels of IL-21, or regulate the induction of cellular subsets that generate IL-21, will provide new therapeutic opportunities to improve immunity to diseases that require CD8+ T cell responses to be controlled, such as chronic viral infection and tumors.
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